Biochemistry and molecular genetics of poly-γ-glutamate synthesis
M Ashiuchi, H Misono - Applied microbiology and biotechnology, 2002 - Springer
M Ashiuchi, H Misono
Applied microbiology and biotechnology, 2002•SpringerCurrent research into poly-γ-glutamate (PGA) and its biosynthesis is reviewed. In PGA-
producing Bacillus subtilis, glutamate racemase supplies abundant DL-glutamate, the
substrate for PGA synthesis. The pgsBCA genes of PGA-producing B. subtilis, which encode
the membrane-associated PGA synthetase complex PgsBCA, were characterized and the
enzyme complex was suggested to be an atypical amide ligase based on its structure and
function. A novel reaction mechanism of PGA synthesis is proposed.
producing Bacillus subtilis, glutamate racemase supplies abundant DL-glutamate, the
substrate for PGA synthesis. The pgsBCA genes of PGA-producing B. subtilis, which encode
the membrane-associated PGA synthetase complex PgsBCA, were characterized and the
enzyme complex was suggested to be an atypical amide ligase based on its structure and
function. A novel reaction mechanism of PGA synthesis is proposed.
Abstract
Current research into poly-γ-glutamate (PGA) and its biosynthesis is reviewed. In PGA-producing Bacillus subtilis, glutamate racemase supplies abundant DL-glutamate, the substrate for PGA synthesis. The pgsBCA genes of PGA-producing B. subtilis, which encode the membrane-associated PGA synthetase complex PgsBCA, were characterized and the enzyme complex was suggested to be an atypical amide ligase based on its structure and function. A novel reaction mechanism of PGA synthesis is proposed.
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